Quantitative estimation of ascorbic acid and related substances in biological extracts by separation on a paper chromatogram
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Quantitative estimation of ascorbic acid and related substances in biological extracts by separation on a paper chromatogram.
In studies on the synthesis of L-ascorbic acid in plants and animals the need arose for a method permitting separation and quantitative estimation of closely related enediol compounds such as D-araboascorbic and L-ascorbic acids. Simple titration methods using 2:6-dichlorophenolindophenol (Harris & Ray, 1935; Birch, Harris & Ray, 1933; Harris & Olliver, 1942) will not differentiate between clos...
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450-5 m,u.)since it yielded a singleband on mixed chromatography with cryptoxanthin obtained from yellow maize (Sadana, 1946). Zone II. This was an orange-coloured band. The pigment was identified as neo-,-carotene U (maxima in light petroleum 480 and 450 mp.; Sadana, 1949). Zone III. This was reddish orange in colour. The pigment was extracted and identified as $-carotene by its absorption spe...
متن کاملDetection of Ascorbic Acid in Biological Samples by a New Modified Glassy Carbon Electrode
The present work describes the construction of a new modified electrode by casting the appropriate mixture of a metallocene, which has been introduced in the experimental part, as a mediator at the surface of glassy carbon (GC) electrode. The proposed modified GC electrode was used for the determination of ascorbic acid (AA) in phosphate buffer (PB) solution (pH = 4.0). When compared to bare GC...
متن کاملBiological synthesis of ascorbic acid: the conversion of derivatives of D-galacturonic acid into L-ascorbic acid by plant extracts.
Broquist, H. P., Kohler, A. R., Hutchison, D. J. & Burchenal, J. H. (1953). J. biol. Chem. 202, 59. Brown, E. G., Goodwin, T. W. & Pendlington, S. (1955). Biochem. J. 61, 37. Buchanan, J. M., Levenberg, B., Flaks, J. G. & Gladner, J. A. (1955). In Amino Acid Metabolism, p. 743. Ed. by McElroy, W. D. & Glass, B. Baltimore: Johns Hopkins Press. Fosse, M. R. (1914). C.R. Acad. Sci., Paris, 158, 10...
متن کاملInhibition of urease by copper salts in the presence of ascorbic acid and related substances.
Elson (1943) found that urease is inhibited by small amounts of ascorbic acid and that this inhibition is eliminated in the presence of cysteine. Quastel (1943) suggested that the inhibition was due to a reaction between dehydroascorbic acid and an -SH group of the enzyme, and that the protective effect of thiol compoundcls was due to the fact that they reduced dehydroascorbic acid. Giri & Sesh...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1953
ISSN: 0306-3283
DOI: 10.1042/bj0550821